Sample preparation for single-cell proteomics

Research articles and protocols:

Minimal sample prep Droplet sample prep Protocol article Videos

Sample preparation in an essential first step for quantitative single-cell protein analysis by mass-spectrometry. These pages contain resources and protocols for automated sample preparation methods compatible with many single-cell proteomics methods:

        Minimal sample preparation page   Droplet sample preparation page

Reviews and perspectives

Highlights Reviews Perspectives

Highlights

• Technology feature at Nature Methods
• Single-cell proteomics for the Human Cell Atlas
• News in Proteomics Research
• The single cell proteomics revolution

Reviews

• Single-cell protein analysis by mass spectrometry, Current Opinion in Chemical Biology
• Single cell protein analysis for systems biology, Essays in biochemistry

Perspectives

• Transformative Opportunities for Single-Cell Proteomics, Journal of Proteome Research
• Unpicking the proteome in single cells, Science

About the methods

These methods were developed in the Slavov Laboratory and SCP Center at Northeastern University. The research was supported by funding from the NIH Director’s Award and by an Allen Distinguished Investigator Award from the Paul G. Allen Frontiers Group.